The first great stimulus to the development of the theory and practice of experimental design came from agricultural research. R.A Fisher realized that current practice in field plot trials failed to produce unambiguous conclusions. This led him from about 1923 onward to examine the principles underlying scientific experimentation and to evolve new techniques of design. Not only was it necessary to devise procedures that would permit design. Not only was it necessary to devise procedures that would permit the drawing of valid inferences from experimental results, but these inferences has to freed as far as possible from the obscuring effect of the variability inherent in the material and the nature of the observations. Not only was randomization needed in order to remove bias, but also for making valid estimates of standard errors. The labour of performing experiments and the number of questions requiring investigation were so great as to make imperative techniques that should use most effectively the materials and effort employed and should give results of high precision. To fisher goes most of the credit for stating and solving these problems and creating a new branch of science from which experimentation in many fields of research has since benefitted. Although this science of experimental design is today used widely in biology and elsewhere, the standard nomenclature retains evidence of its agricultural origin.

Some of its main topics are:

1. Randomized blocks vs Latin square
2. Latin square
3. Computation of trend values
4. Trends

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